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Ariya Lauretti Noti Pdf
Posted:adminJul 20, 1976 - blanc, Paolo Lauretti, Jean-Philippe Lemoine, Yves. Folder transfer 4 crack. Maria-Sube, Jimmy. Koberto under Persian spell (notice husband^s wink!) 3.
RELATED APPLICATIONS The present patent document claims the benefit of the filing date under 35 U.S.C. §119(e) of Provisional U.S. Patent Application Ser. 60/903,116, filed Feb. 23, 2007, which is hereby incorporated by reference. FIELD The present invention relates to the field of diagnostics. More particularly, the invention disclosed herein is directed to methods, compositions, and kits for detecting nucleic acids, polynucleotides, pathogens, toxins, or other agents or factors that are desirably detected or measured.
BACKGROUND There is a great need to detect and quantify various molecular species, such as polynucleotides, polypeptides, carbohydrates, lipids, and small molecules. For example, current methods of detecting a polynucleotide, such as those associated with pathogens, pathogen infection, human genes associated with diseases and disorders, altered physiology or physiological conditions, genetically modified organisms (GMOs, i.e., organisms with transgenic DNA), biowarfare agents, veterinary applications, and agricultural applications presently rely on complex methods, such as the polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), or branched DNA (bDNA). These methods require skilled personnel and specialized equipment. Further, the methods are generally incapable of determining the presence or quantity of polynucleotides in crude cell and tissue extracts. There are similar difficulties in the existing immunoassays for detecting antigens. In formula (II), independently at each occurrence, R 1 and R 2 are each independently selected from C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl; R 3 is selected from the group consisting of hydrogen, C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, carbonyl, sulfinyl, sulfonyl, and amino groups; n is 1 or 2; R 4 and R 9 are each independently selected from the group consisting of H, C 1-C 6 alkyl, C 2-C 6 alkenyl, C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, halo, carbonyl, sulfinyl, sulfonyl, and amino groups. In a further embodiment, the dye is a compound that is represented by the formula (III), or a salt or ester thereof.
Where X- is an anion. More preferably, the anion is a halogen; yet more preferably, the anion is iodide.
In one embodiment of formula (I, II, and III), n is 0. In another embodiment of formula (I, II, and III), n is 1.
In another embodiment of formula (I, II, and III), n is 2. In yet another embodiment of formula (I, II, and III), n is 3. In one embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, aryl, and heteroaryl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, and alkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently alkyl.
In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of alkyl, alkenyl, and alkynyl. In one embodiment of formula (I, II, and III), R 3 is alkyl. The compounds in the following list are within the scope of formulas (I-III.
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Jul 20, 1976 - blanc, Paolo Lauretti, Jean-Philippe Lemoine, Yves. Folder transfer 4 crack. Maria-Sube, Jimmy. Koberto under Persian spell (notice husband^s wink!) 3.
RELATED APPLICATIONS The present patent document claims the benefit of the filing date under 35 U.S.C. §119(e) of Provisional U.S. Patent Application Ser. 60/903,116, filed Feb. 23, 2007, which is hereby incorporated by reference. FIELD The present invention relates to the field of diagnostics. More particularly, the invention disclosed herein is directed to methods, compositions, and kits for detecting nucleic acids, polynucleotides, pathogens, toxins, or other agents or factors that are desirably detected or measured.
BACKGROUND There is a great need to detect and quantify various molecular species, such as polynucleotides, polypeptides, carbohydrates, lipids, and small molecules. For example, current methods of detecting a polynucleotide, such as those associated with pathogens, pathogen infection, human genes associated with diseases and disorders, altered physiology or physiological conditions, genetically modified organisms (GMOs, i.e., organisms with transgenic DNA), biowarfare agents, veterinary applications, and agricultural applications presently rely on complex methods, such as the polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), or branched DNA (bDNA). These methods require skilled personnel and specialized equipment. Further, the methods are generally incapable of determining the presence or quantity of polynucleotides in crude cell and tissue extracts. There are similar difficulties in the existing immunoassays for detecting antigens. In formula (II), independently at each occurrence, R 1 and R 2 are each independently selected from C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl; R 3 is selected from the group consisting of hydrogen, C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, carbonyl, sulfinyl, sulfonyl, and amino groups; n is 1 or 2; R 4 and R 9 are each independently selected from the group consisting of H, C 1-C 6 alkyl, C 2-C 6 alkenyl, C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, halo, carbonyl, sulfinyl, sulfonyl, and amino groups. In a further embodiment, the dye is a compound that is represented by the formula (III), or a salt or ester thereof.
Where X- is an anion. More preferably, the anion is a halogen; yet more preferably, the anion is iodide.
In one embodiment of formula (I, II, and III), n is 0. In another embodiment of formula (I, II, and III), n is 1.
In another embodiment of formula (I, II, and III), n is 2. In yet another embodiment of formula (I, II, and III), n is 3. In one embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, aryl, and heteroaryl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, and alkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently alkyl.
In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of alkyl, alkenyl, and alkynyl. In one embodiment of formula (I, II, and III), R 3 is alkyl. The compounds in the following list are within the scope of formulas (I-III.
...">Ariya Lauretti Noti Pdf(23.12.2018)Jul 20, 1976 - blanc, Paolo Lauretti, Jean-Philippe Lemoine, Yves. Folder transfer 4 crack. Maria-Sube, Jimmy. Koberto under Persian spell (notice husband^s wink!) 3.
RELATED APPLICATIONS The present patent document claims the benefit of the filing date under 35 U.S.C. §119(e) of Provisional U.S. Patent Application Ser. 60/903,116, filed Feb. 23, 2007, which is hereby incorporated by reference. FIELD The present invention relates to the field of diagnostics. More particularly, the invention disclosed herein is directed to methods, compositions, and kits for detecting nucleic acids, polynucleotides, pathogens, toxins, or other agents or factors that are desirably detected or measured.
BACKGROUND There is a great need to detect and quantify various molecular species, such as polynucleotides, polypeptides, carbohydrates, lipids, and small molecules. For example, current methods of detecting a polynucleotide, such as those associated with pathogens, pathogen infection, human genes associated with diseases and disorders, altered physiology or physiological conditions, genetically modified organisms (GMOs, i.e., organisms with transgenic DNA), biowarfare agents, veterinary applications, and agricultural applications presently rely on complex methods, such as the polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), or branched DNA (bDNA). These methods require skilled personnel and specialized equipment. Further, the methods are generally incapable of determining the presence or quantity of polynucleotides in crude cell and tissue extracts. There are similar difficulties in the existing immunoassays for detecting antigens. In formula (II), independently at each occurrence, R 1 and R 2 are each independently selected from C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl; R 3 is selected from the group consisting of hydrogen, C 1-C 6 alkyl, C 2-C 6 alkenyl, and C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, carbonyl, sulfinyl, sulfonyl, and amino groups; n is 1 or 2; R 4 and R 9 are each independently selected from the group consisting of H, C 1-C 6 alkyl, C 2-C 6 alkenyl, C 2-C 6 alkynyl, C 6-C 10 aryl, hydroxyl, alkoxy, halo, carbonyl, sulfinyl, sulfonyl, and amino groups. In a further embodiment, the dye is a compound that is represented by the formula (III), or a salt or ester thereof.
Where X- is an anion. More preferably, the anion is a halogen; yet more preferably, the anion is iodide.
In one embodiment of formula (I, II, and III), n is 0. In another embodiment of formula (I, II, and III), n is 1.
In another embodiment of formula (I, II, and III), n is 2. In yet another embodiment of formula (I, II, and III), n is 3. In one embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, aryl, and heteroaryl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently selected from group consisting of alkyl, alkenyl, and alkynyl. In another embodiment of formula (I, II, and III), R 1 and R 2 are each independently alkyl.
In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl. In one embodiment of formula (I, II, and III), R 3 is selected from the group consisting of alkyl, alkenyl, and alkynyl. In one embodiment of formula (I, II, and III), R 3 is alkyl. The compounds in the following list are within the scope of formulas (I-III.
...">Ariya Lauretti Noti Pdf(23.12.2018)